Activity Differences of Cellulase in Different Organs and Subcellular Structures of Camellia sinensis
Cellulase,a kind of important hydrolase,is critical for growth and development and metabolism of plant.In order to understand the activity characteristics of cellulase in Camellia sinensis,the activity of the three main cellulasees of endo-glucanase,exo-glucanase and β-glucosidase in different organs and subcellular structures in Fuzao NO.2 were studied.The results indicated that the activity of the endo-glucanase in different position of tea ‘four and a bud’ was increased with leaf maturity development,showed that the fourth leaf ＞ the third leaf ＞ the second leaf ＞ the first leaf ＞ bud,which was contrary to the activity of β-glucosidase.The activity of exo-glucanase and β-glucosidase showed that had a lowest level in the fourth leaf.The activity of endo-glucanase had a highest level in tea flower,however exo-glucanase and β-glucosidase in tea leaf and pericarp respectively.The activity of endo-glucanase and β-glucosidase in cytoplasmic proteins is higher than in membrane proteins,however,the activity of exo-glucanase couldn't be detected in cytoplasmic proteins and membrane proteins.
国家科技期刊平台
NSTL
万方数据
维普
