Morphological Diversity and Ploidy Identification in Agave Germplasms
Ten morphological traits including leaf color,have/none marginal spine,apical spine type,leaf length,leaf width,length/width of leaf,thickness of leaf base,length of apical spine,and spine basal width were examined,and ploidy level was determined by an analysis of flow cytometry.SPSS statistical analysis for leaf length,leaf width,length/width of leaf,thickness of leaf base,length of apical spine,spine basal width revealed that The coefficient of variation within Agave germplasms was 0.9％-13.33％,1.69％-9.13％,1.77％-7.61％,3.99％-11.62％,7.82％-14.31％,9.08％-14.84％,among Agave germplasms was 21.60％,13.95％,15.9％,21.48％,36.59％,30.77％ respectively.ANOVA showed that ten morphological traits were statistically different among 25 Agave germplasms (α=0.05),but no different within germplasm (α＞0.05).Cluster analysis based on complete distance with SAS of ten morphological traits showed that 25 germplasms were divided into three groups,A.americana L,Guangxi76416,Nanya 1,ML387,A.tequilana F.A.C weber,A.sisalana perrine,and Yuexi75 were scattered into the first group,Guifu4,H.11648,Dong74,Dong27,Dong26,Dongl6 were clustered into the second group,the other 12 germplasms were scattered into the third group.The principal component analysis results indicated that the sum of eigenvalue of the first 4 principal components was 5.84,and the contribution rate accounted for 97.29％.Among the 25 Agave germplasms,14 germplasm such as H.11648 and Dong2 are diploids,Dong16 and Guangxi76416 are triploids,5 germplasms such as Yuexi75 and Yuexi 114 are tetraploids,Ken3 and A.sisalana Perrine are pentaploids,Yuexi74 is a hexaploid,A.americana L.is a mixoploid of pentaploid and hexaploid.The results would provide basic reference on Agave germplasm identification,and classification.
国家科技期刊平台
NSTL
万方数据
维普
