Optimization of High Frequency Embryo Induction System for Isolated Microspore Culture in Chinese Cabbage
To optimize the system of high frequency embryo induction for free microspore culture in Chinese cabbage,a total of 20 different genotypes were used as the experimental materials to study the factors that affecting the embryo induction of free microspore in Chinese cabbage and plantlet regeneration.The results showed that a total of 14 genotypes produced microspore embryo,significant differences in rate of embryo induction were observed among these genotypes,indicating that genotype was the main factor affecting the formation of microspore embryo.The rate of free microspore embryo induction was significantly improved by the optimization of high frequency embryo induction system for free microspore culture in Chinese cabbage.The optimal combination was the following:bud collected in the period of flowering,a ratio of petal length and anther length ranged from 0.5 to 0.75,bud in 4 ℃ condition for 1 d,then the bud in NLN culture medium containing 150 g/L sucrose and a heat shock conducted under 33 ℃ for 2 d.Finally,the bud was transferred into the culture medium containing 130 g/L sucrose after centrifugation.The medium containing 0.1 mg/L 6-BA and 0.5 mg/L NAA could promote the microspore embryogenesis.It is beneficial for the formation of embryos by adding 0.5 g/L activated carbon.
Chinese cabbageisolated microsporeembryogenotypeoptimization
国家科技期刊平台
NSTL
万方数据
维普
