Effects of Long-term Application of Nitrogen Fertilizer on Soil and Endophytic Fungal Compositions in Rhizospheres and Roots of Sug-arcanes
To develop sustainable sugarcane production and scientific use of nitrogen fertilizer,the effect of long-term application of nitrogen fertilizer on the soil and endophytic fungal compositions in the rhizosphere and roots were ex-plored.The soil and endophytic fungal compositions in the rhizospheres and roots of sugarcanes under long-term appli-cation of nitrogen fertilizers were analyzed.0,96,482,964 kg/hm2 nitrogen fertilizer application(CK,L,M,H)were set in the same sugarcanes field,and based on the high-throughput sequencing techniques,the soil and endophytic fungal compositions in the rhizospheres and roots of sugarcanes were analyzed.In comparison with CK,although the richness of soil fungi in the rhizospheres of sugarcanes was significantly increased by different nitrogen application levels(P<0.05),particularly,L,M and H treatments increased by 39.58%,24.04%and 35.33%,respectively;the diversity was not significantly different.Meanwhile,there were not significantly different in the diversity and richness of the endo-phytic fungi in the sugarcane roots under different nitrogen application treatments.Moreover,the soil fungi in the rhizospheres of sugarcanes,the relative abundances of Saitozyma,Trichoderma,unclassified_p__Ascomycota and Fusa-rium increased under nitrogen application.On the contrary,the relative abundances of Talaromyces,unclassi-fied_f__Herpotrichiellaceae,Coniosporium,Penicillium,Chaetosphaeria,Chaetomium,unclassified_o__Hypocreales decressed under nitrogen application.In addition,the endophytic fungi in sugarcane roots,unclassified_k__Fungi and unclassified_p__Ascomycota increased,and Talaromyces,Chaetomium,unclassified_f__Strophariaceae decreased under different nitrogen application treatments.The results showed that the enrichment of single soil fungal communities in the rhizospheres or roots,and the potential risk of decreasing cane resistance could be induced by long-term nitrogen fertilizer application only.Meanwhile,in view of maintaining the soil health in the rhizospheres of sugarcanes,the op-timal nitrogen application rate for sugarcane was 96 kg/hm2.
万方数据
维普
