The sisal zebra disease,caused by fungal pathogen Phytophthora nicotianae Breda,is one of the main dis-eases in sisal,which seriously affects the development of sisal industry.The pathogenic mechanism is not clear up to now.In order to obtain new anti-zero disease sisal variety,Yuexi 114 and H.1 1648 were used as the parents to breed new offsprings.Rema No.1 is one of the backcross progenies of Yuexi 114 and H.1 1648.Morphological traits including leaf shape,leaf color,have/none marginal spine,apical spine type,leaf length,leaf width,thickness of leaf base,length of apical spine,and spine basal width,plant height,inflorescence shape and flower stalk height,number of pedical,flower,fruit and seed characters,number of leaf,fiber ratio were examined.Ploidy level,genome size and resistance to P.nico-tianae Breda was determined by an analysis of wall removal and low permeability flame drying method,flow cytometry,and inoculating P.nicotianae Breda in field and lab respectively.Rema No.1 is characterized by the presence of gray green,fleshier and rigid,sword-leaf with wax powder on the leaf surface,1.2-2.0 cm apical spine,none marginal spine.Leaf length,leaf width and leaf base is about 130 cm,16 cm,and 3.5 cm,respectively.The leaves rise in a spiral ar-rangement around the stem.Rema No.1 is also characterized by plant height 1.8-2.5 m,flower stalk 3-7 m,paniculate inflorescence,with 20-32 pedicals,complete flower,yellow flower with 6 petals,oblong capsule,dark brown to almost black upon maturity,lunate black seeds,marginate.leaves 360-400,leaf fiber ratio 3.5％,fiber length 100 cm.The life span is 6-9 years.Rema No1 is tetraploid(2X=4n),chromosome number is between 104-118,the genome size of Rema No.1 is 2C=11.69 pg.Rema No.1 is high resistant to Phytophthora nicotianae Breda,and would be the main cultures in high incidence area of zero leaf disease in sisal planting region.
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