Identification and Expression Profile Analysis of KAS Gene Family in Camellia oleifera
Beta-ketoacyl-acyl carrier protein synthase(KAS)is the key enzyme system in plant fatty acid biosynthesis.In order to investigate the function of the KAS gene family in the maturation and oil accumulation of Camellia oleifera,this study used bioinformatics methods and analyzed ther physicochemical properties,chromosomal localization,sub-cellular localization,secondary structure,phylogenetic trees,conserved motifs,promoter cis-acting elements,and RT-qPCR to analyze the expression patterns in the oil rapid accumulation stage of C.oleifera.The findings indicated that 14 Camellia oleifera KAS family members were distributed on seven chromosomes with relative molecular masses between 1.13 kDa and 5.15 kDa,and most of them were acid-stabilized proteins.Except for CoKAS II-2,which was localized in mitochondria and chloroplasts,all of them were localized in chloroplasts.Phylogenetic analysis revealed that CoKAS was divided into three subclasses,with similarities in structure and conserved motifs among KAS family numbers in the same subfamily.The promoter regions of the CoKAS gene family members were enriched with the cis-acting ele-ments growth and development-related,light response,hormone induction,and adversity response.Expression analysis revealed that most of the CoKAS genes were highly expressed during the oil rapid accumulation stage.In addition,it was found a strong correlation between the members of the CoKAS and CoMYB gene families during fruit ripening in C.oleifera.The findings would provide a theoretical basis for comprehensively analyzing the function of CoKAS genes.
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