Genetic Diversity Studies on Aquilaria sinensis Based on Genome Re-sequencing
Knowledge on germplasm resource information is the foundation for the genetic improvement of Aquilaria sinensis,and genome resequencing is a powerful tool for analyzing the genetic diversity of germplasm resources.How-ever,there is no research on the genetic diversity of A.sinensis using genome resequencing.Based on the collection of A.sinensis germplasm resources in the early stages,this study utilized genome resequencing to obtain 1284 Gb of rese-quencing data from 60 samples of A.sinensis.Using the agarwood genome,6 716 166 high-quality variation sites were detected,including 6 284 344 SNP sites and 428 658 Indel sites.Principal component analysis,phylogenetic analysis,and genetic structure analysis all indicated that A.sinensis could be divided into three distinct groups(Pop1,Pop2 and Pop3),with two of the groups further subdivided into multiple subgroups.Population selection signals were detected in all the groups,with Pop3 showing the highest frequency of selection signals.However,nucleotide polymorphism calcu-lations indicated that Pop3 had the lowest nucleotide polymorphism.This study would provide valuable information for exploring the genetic resource diversity of A.sinensis and lay a theoretical foundation for genome-based genetic breed-ing of A.sinensis.