Creation and Application of Big Rosette Leaf Angle and Mutiple Tillers Material of Chinese Cabbage
To accelerate breeding of small-sized,high-density planting Chinese cabbage varieties,multi-tiller non-heading Chinese cabbage and flowering Chinese cabbage varieties,thereby reforming the planting mode to improve yield and quality of Chinese cabbage,conventional hybridization methods were used for three parents hybridization in order to create new germplasm materials by using Brassica rapa pekinensis,Brassica rapa parachinensis,Brassica rapa purpurea as materials.At the same time,a 6-generation genetic population was constructed by crossing the newly created Chinese cabbage'21M-193'with the Chinese cabbage'Baiyang'to study the inheritance and application of the rosette leaf angle and tiller in Chinese cabbage.The results showed that'21M-193'created by multiple-parent hybridization exhibited the characteristics of big leaf angle and multiple tillers.Measurement data indicated that the average leaf angle of'21M-193'was 82.7°,while'Baiyang'had an average leaf angle of 30.5°.The average leaf angle of F,hybrid of'21M-193'and'Baiyang'was 40.7°,leaning towards'Baiyang',displaying a small leaf angle phenotype.Among F2,the leaf angles showed a normal distribution with few extreme individuals below 10° and above 84°,and the majority of intermediate types between 30° and 50°.The segregation ratio did not conform to Mendelian inheritance laws,suggesting that the inheritance of leaf angle of Chinese cabbage rosette leaf was a quantitative trait controlled by multiple genes.Additionally,it was found that'21M-193'had 28 tillers per plant,while'Baiyang'had 7 tillers per plant.The tiller number of F,hybrid of'21M-193'and'Baiyang'was 18 tillers per plant,which was intermediate level of'21M-193'and'Baiyang',leaning towards'21M-193'.The above research results not only create a new germplasm of Chinese cabbage with big leaf angle and multiple tillers,but also preliminarily reveal the genetic laws of leaf angle and tiller traits in Chinese cabbage.It also provides valuable materials and data support for gene mapping and molecular assisted breeding of these two important agronomic traits,which will be of great significance for promoting the improvement of varieties and innovation of high-yield and high-quality cultivation modes of Chinese cabbage.
Chinese cabbageleaf angletillerinheritancegermplasm creationgene localizationquantitative trait
万方数据
维普
