Breeding for Three Lines of Nucleo-cytoplasmic Interaction Male Sterility in Pepper
In order to solve the problems of high cost and difficulty in ensuring seed purity in artificial castration of pepper and promote the healthy development of the pepper industry,the breeding for three lines of nucleo-cytoplasmic interaction male sterility in pepper was carried out.Firstly,sterile plants were identified from the progeny of pepper with the code 149.Then,through cross breeding,back cross breeding and selfing breeding methods,the pepper sterile line BC8149A-2,maintainer line F10149B-2 and restorer line WYS-1 were breeded.Through the stability test of the hybrid effect of the sterile line and the maintainer line,the maintainer line F10149B-2 achieved a 100%restoration effect on the sterility of the sterile line BC8149A-2,which were a qualified pair of sterile and maintainer lines.According to the determination of yield combining ability of sterile line BC8149A-2 and restorer line WYS-1,the hybrid yield combining ability of sterile line BC8149A-2 and restorer line WYS-1 showed the best performance,both significantly higher than the control'Hajiao No.6'.The acquisition of the three lines has improved seed production efficiency,has reduced seed production costs and has solved the problem of low seed purity caused by incomplete manual emasculation,providing a reference for the breeding for three lines of nucleo-cytoplasmic interaction male sterility in pepper.