Effects of Different Exogenous Hormones on Physiological Characteristics of Seedlings Leaves of Jerusalem artichoke
To investigate the effects of different exogenous substances on the physiological characteristics of seedlings leaves of Jerusalem artichoke and explore the changes in physiological characteristics during the growth process of Jerusalem artichoke,variety of'Qingyu No.1'was used as the experimental material,and the seedlings of'Qingyu No.1'were treated with 200 mmol/L abscisic acid(ABA),100 mmol/L methyl jasmonate(MeJA),100 mmol/L gibberellin(GA3)and 200 mmol/L salicylic acid(SA),respectively.Samples were taken at 24,48 and 72 hours after treatment to determine the contents of reduced glutathione(GSH),ascorbic acid(AsA),malondialdehyde(MDA),soluble protein,soluble sugar and hydrogen peroxide(H2O2)in the leaves of the seedlings,as well as the activities of superoxide dismutase(SOD),ascorbate peroxidase(APX)and catalase(CAT).The results showed that ABA,MeJA,GA3 and SA treatments could all extremely significantly increase the H2O2 content and APX activity in the leaves of Jerusalem artichoke seedlings within 72 h,extremely significantly decreasing the soluble sugar content and GSH content,and significantly increasing the soluble protein content(except for ABA treatment for 24 h).In addition,the SOD activity in Jerusalem artichoke leaves increased significantly than that of CK(leaves not treated with hormones)after ABA treatment and the content of AsA was extremely significantly lower than that of CK after MeJA treatment.Under GA3 treatment,the activity of SOD was extremely significantly lower than that of CK,and under SA treatment,the content of MDA was extremely significantly higher than that of CK,and the activity of SOD was significantly lower than that of CK.The results laid a theoretical foundation for the subsequent breeding of drought resistant varities of Jerusalem artichoke and the expansion of Jerusalem artichoke planting areas.
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