Characterization of Bacterial Communities Associated with the Intestinal Tracts and Culture Environment of Sea Cucumber (Apostichopus japonicus) in Different Culture Seasons
The aim of the study was to use a combination of denaturing gradient gel electrophoresis tech-nology to evaluate the bacterial communities of artificial diet-fed sea cucumber (Apostichopus japonicus) adults in different culture seasons .Overall ,the results showed that bacterial communities associated with sea cucumbers were considerable stable in different culture seasons ,little differences in the bacterial as-semblages .Phylogenetic analysis demonstrated that the most frequently observed species were γ-Pro-teobacteria and α-Proteobacteria ,additional species were β-Proteobacteria ,ε-Proteobacteria ,Spirochaet-ales ,Bacteroidetes ,Cytophagales and Chlamydiales groups .The dominant bacterial community composi-tion including V ibrio sp ., Roseobacter sp ., Photobacterium sp . in intestine samples ;V ibrio sp ., Ro-seobacter sp .,Cytophage sp .was the dominant group in the seawater samples ;while Vibrio sp .,Micro-coccus sp .,Pseudoalteromonas sp .,Photobacterium sp .,Chloroflexi sp .,Shewnella sp .,Pantoea sp ., X anthomonas sp .and ,A grobacterium sp .were the dominant group in the sediment samples .These find-ings provide the sequence foundation for the development of molecular assays that target sea cucumber bac-teria and probiotics .
denaturing gradient gel electrophoresisbacterial communityApostichopus japonicas
NETL
NSTL
万方数据
维普
