Effect of Imidazole Compounds KK-42 on Immunologic Functions of Freshwater Prawn Macrobrachium nipponense
The expressions of cytMnSOD,mtMnSOD and Hemocyanin genes and changes in corresponding enzyme activities were investigated in intermolt freshwater prawn Macrobrachium nipponense exposed to imidazole compounds KK-42 at a concentration of 0(control)and 1.95×10-4mol/L(treatment)in 3 h,6 h,12 h,24 h and 48 h and the prawn in two groups were injected individually with 20 μL of Aeromonas hydrophila into the ventral sinus 12 h post KK-42 treatment to evaluate the effect of KK-42 on expression of genes related to immune and antibacterial ability in the freshwater prawn.The results showed that the mRNA expression of cytMnSOD,mtMnSOD and Hemocyanin genes was increased significantly in 3,6 and 12 h after KK-42 treatment,and the SOD activity and hemocyanin content were also significantly increased from 6 h to 48 h,with the maximum in 24 h.The lower mortality was observed in the prawn infected with A.hydrophila exposed to KK-42 pretreatment.The findings indicated that KK-42 induced genes related to immune expression,and that KK-42 pretreatment enhanced the antibacterial ability in the praw n.
NETL
NSTL
万方数据
维普
