Function of Crustin Gene in Regulation of Intestinal Microbiota Homeostasis in Ridgetail White Prawn Exopalaemon carinicauda
In order to investigate the function of crustin in regulation of intestinal microbiota homeostasis in ridgetail white prawn Exopalaemon carinicauda,the intestinal microbiota characteristics were investi-gated in the ridgetail white prawn with body length of(5.47±0.31)cm with 16S rRNA sequencing tech-nology.The results showed that there was significant change in composition of intestinal microflora in the ridgetail white prawn exposed to EcCrustin2 gene knockdown,without significant difference in α-diversity between the control and siEcCrustin2 gene groups.Proteobacteria,Tenericutes and Firmicutes were found to be the dominant phyla in the intestinal microbiota in the ridgetail white prawn exposed to EcCrustin2 gene knockdown at the phylum level.The relative abundance of Proteobacteria was increased in siEcCrus-tin2 group,and those in Tenericutes and Firmicutes were decreased.Photobacterium and Vibrio were the dominant bacterial genera in the intestinal microbiota of E.carinicauda group at the genus level,with significant de-crease in relative abundance of Photobacterium,and the significant increase in abundance of Vibrio in siEcCrustin2 group.Photobacterium damselae subsp.damselae was shown to be the dominant species,with significantly elevated abundance after EcCrusin2 gene knockdown at the species level.Functional prediction by Tax4Fun revealed that intestinal microbiota were involved in carbohydrate metabolism,lipid metabolism,infectious diseases,cell motility and membrane transport more apparently in the RNAi group than those in control group.There was close relationship between the EcCrustin2 and the intestinal microflora in E.carinicauda group.The findings will contribute to comprehensive understanding of the composition and plasticity of the shrimp microbiota,which will provide innovative strategies for promoting the development of aquaculture industry.
万方数据
维普
