首页|福建养殖皱纹盘鲍线粒体基因组全测序及分析

福建养殖皱纹盘鲍线粒体基因组全测序及分析

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为有效鉴别鲍的种类,探讨其来源,通过高通量测序获得福建养殖皱纹盘鲍线粒体基因组全序列,分析比较其序列与GenBank中收录的3个皱纹盘鲍线粒体基因组全序列或近全序列.试验结果显示,皱纹盘鲍线粒体基因组全长17 037 bp,具有37个编码基因,包括13个蛋白质编码基因、22个tRNA基因和2个rRNA基因.除控制区存在碱基差异外,当前4个皱纹盘鲍线粒体全序列基本一致.基于鲍属线粒体基因组全序列构建系统发育树,4个皱纹盘鲍聚为一支,再与红鲍、黑鲍及黑足鲍聚为一支,其中福建养殖皱纹盘鲍连江群体与韩国巨济群体关系较近.笔者分析了不同皱纹盘鲍群体间的线粒体碱基组成和鲍属贝类系统进化关系,试验结果丰富了皱纹盘鲍遗传信息库,可为东南沿海鲍种的鉴定和良种选育提供参考.
Sequencing and Analysis of Complete Mitochondrial Genome of Disk Abalone Haliotis discus hannai Cultured in Fujian Province
In order to effectively identify abalone species and explore the sources,the complete mitochon-drial genome of disk abalone Haliotis discus hannai cultured in Fujian province was sequenced by high-throughput sequencing technologies.The sequences obtained were preliminarily analyzed and the whole ge-nome was compared with three complete or nearly-complete mitochondrial genome sequence of the disk ab-alone deposited in GenBank.The results showed that the disk abalone had mitogenome of 17 037 bp in to-tal length with 37 genes identified including 13 protein coded,22 tRNA and 2 rRNA genes.Except for the nucleotide bases differences in the control region,basically the same complete or nearly-complete mito-chondrial sequences were observed in the four populations of the disk abalone.A phylogenetic tree con-structed based on reported complete mitochondrial genome sequences of species in Haliotis genus revealed that all disk abalone were clustered with each other first and then clustered with H.ru fescens,H.crach-erodii and H.iris.The disk abalone from Lianjiang,China,had a close relationship with those from Geo-je,Republic of Korea.In brief,sequencing and analysis of the entire mitochondrial genome of the abalone cultivated in Fujian province enriched the genetic information database of this species.The finding provides reference for the identification and breeding of high-quality abalone varieties in the southeastern coastal wa-ters of China.

Haliotis discus hannaimitochondrial genomephylogenyhigh-throughput sequencing

王伟

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福州市海洋与渔业技术中心,福建 福州 350007

福州海洋研究院,海洋与渔业技术研究中心,福建 福州 350108

皱纹盘鲍 线粒体基因组 系统发育 高通量测序

2025

水产科学
辽宁省水产学会

水产科学

北大核心
影响因子:0.97
ISSN:1003-1111
年,卷(期):2025.44(1)