为探究耳石微化学标记技术在淞江鲈增殖放流中的应用,在水温(28.0±3)℃下,在三级过滤的养殖水体中添加SrCl2·H2O,将质量浓度分别调至0、12、18、36、72 mg/Ⅰ,标记7 d继续常规饲养,探究不同质量浓度的锶对体长(8.34±0.77)cm、体质量(7.89±2.57)g的淞江鲈耳石锶标记效果的影响.试验结果表明:不同质量浓度的锶标记对淞江鲈死亡率和生长影响不明显;外源锶质量浓度与耳石边缘的Sr/Ca值之间存在明显的线性关系,随着锶质量浓度的增加,标记后的Sr/Ca值逐渐增高,标记效果显著增加;随着标记后时间的变化,肌肉和鳃中的锶元素含量呈先升后降的趋势,肌肉中的锶残留可在标记后30 d内代谢完全.锶标记原理和方法在淞江鲈的大规模放流群体标记中可靠和安全,建议选择36 mg/L作为外源锶的最佳标记质量浓度,标记持续时间为7 d.
Strontium Labeling Technique of Otolith in Roughskin Sculpin Trachidermus fasciatus Based on Immersion Method
In order to probe the application of otolithic microchemical labeling technology in breeding and release of roughskin sculpin Trachidermus fasciatus,roughskin sculpin with body length of(8.34±0.77)cm and body weight of(7.89±2.57)g was reared in the third stage filtrated water containing SrCl2·H2O at concentrations of 0,12,18,36 and 72 mg/L for 7 days at water temperature of(28.0±3)℃ to explore the effect of different concentrations of strontium on the strontium labeling effect of otolites in roughskin sculpin.The results showed that there was no significant difference in mortality and growth in roughskin sculpin exposed to different concentrations of strontium labeling.There was an obvious linear relationship between the concentration of exogenous strontium and the ratio of Sr/Ca at the edge of otolith.With the increase in strontium concentration,the labeled Sr/Ca value was gradually increased,and the labeling effect increased significantly.The contents of strontium in muscle and gill were found to be a trend of first increasing and then decreasing with the change of time after labeling,with complete metabolism of the re-sidual strontium in muscle within 30 days after labeling.The findings indicated that the strontium labeling principle and method were reliable and safe in the large-scale release population of roughskin sculpin.It was recommended that 36 mg/L be of the optimal labeling dose for exogenous strontium for 7 days labeling period.
万方数据
维普
