Influence of Elemental Form,Water Temperature,and Body Weight on Iron Accumulation in Sea Cucumber Apostichopus japonicus
Juvenile sea cucumber Apostichopus japonicus with body weight of 60 g and 200 g were placed in a semi-flowing temperature-controlled glass tank in the room,and exposed to the iron concentration of 0.50 mg/L in water from amino acid-chelated iron and ferrous sulfate at water temperature of 15 ℃ and 22 ℃,respectively,to investigate the effects of iron form,water temperature and body weight on the enrich-ment of iron in sea cucumber.The results showed that iron form significantly impacted iron enrichment in sea cucumber at iron concentration of 0.50 mg/L.The sea cucumber exposed to amino acid-chelated iron contained iron concentration of 12.72 mg/kg in their body walls,while those exposed to ferrous sulfate contained 48.27 mg/kg in 8 day exposure to iron-enriched seawater at 22 ℃,indicating that inorganic fer-rous sulfate was more effective than organic amino acid-chelated iron for iron enrichment in sea cucumber.Furthermore,there was more accumulative iron in the sea cucumber at 22 ℃ than that at 15 ℃,without significant difference in iron accumulation capacity in the sea cucumber with different body weight.These findings provide valuable insights for the cultivation of iron-rich sea cucumber.