首页|白及叶枯病病原菌分离鉴定与致病性测定

白及叶枯病病原菌分离鉴定与致病性测定

扫码查看
为明确白及叶枯病病原菌种类,对白及叶枯病病原进行分离、纯化及rDNA-ITS序列分析鉴定其种属,并将纯化病原菌回接于白及植株,评价其致病性。结果表明,从白及患病样品上共分离6株真菌,其中有三株镰刀菌,分别为角化镰刀菌(Fusarium keratoplasticum)、尖孢镰刀菌(Fusarium oxysporum)、木贼镰刀菌(Fusarium equiseti);一株曲霉菌,为日本曲霉(Aspergillus japonicus);一株灰霉,为灰葡萄孢霉(Botrytis cinerea);一株毛霉,为易脆毛霉(Mucor fragilis)。经致病性测定,发现上述6株真菌对白及均有致病性。
Isolation,identification and pathogenicity determination of pathogen of Bletilla Striata leaf blight
In order to clarify the pathogenic bacteria species of Bletilla Striata leaf blight,the pathogen of B.Striata leaf blight was isolated,purified and identified by rDNA-ITS sequence analysis,the purified pathogen was grafted to B.Striata plants to evaluate its pathogenicity.The results showed that a total of 6 fungi strains were isolated from the diseased samples of B.striata,including three strains of Fusarium,namely:F.keratoplasticum,F.oxysporum and F.equiseti;a strain of Aspergillus japonicus;a strain of Botrytis cinerea;a strain of Mucor fragilis.According to the pathogenicity test,it was found that the above 6 strains of fungi were all pathogenic to B.Striata.

Bletilla Striataleaf blightpathogen identificationpathogenicity

刘培琴、姜丽琼、卢麒宇、刘继、谢永东、浣杰、李思言、郑雯雪、李文俊

展开 >

成都市农林科学院,四川成都 611130

白及 叶枯病 病原鉴定 致病性

2024

四川林业科技
四川省林学会 四川省林业科学研究院

四川林业科技

影响因子:0.452
ISSN:1003-5508
年,卷(期):2024.45(3)