In order to clarify the pathogenic bacteria species of Bletilla Striata leaf blight,the pathogen of B.Striata leaf blight was isolated,purified and identified by rDNA-ITS sequence analysis,the purified pathogen was grafted to B.Striata plants to evaluate its pathogenicity.The results showed that a total of 6 fungi strains were isolated from the diseased samples of B.striata,including three strains of Fusarium,namely:F.keratoplasticum,F.oxysporum and F.equiseti;a strain of Aspergillus japonicus;a strain of Botrytis cinerea;a strain of Mucor fragilis.According to the pathogenicity test,it was found that the above 6 strains of fungi were all pathogenic to B.Striata.