四川林业科技2024,Vol.45Issue(3) :142-146.DOI:10.12172/202311140002

白及叶枯病病原菌分离鉴定与致病性测定

Isolation,identification and pathogenicity determination of pathogen of Bletilla Striata leaf blight

刘培琴 姜丽琼 卢麒宇 刘继 谢永东 浣杰 李思言 郑雯雪 李文俊
四川林业科技2024,Vol.45Issue(3) :142-146.DOI:10.12172/202311140002

白及叶枯病病原菌分离鉴定与致病性测定

Isolation,identification and pathogenicity determination of pathogen of Bletilla Striata leaf blight

刘培琴 1姜丽琼 1卢麒宇 1刘继 1谢永东 1浣杰 1李思言 1郑雯雪 1李文俊1
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作者信息

  • 1. 成都市农林科学院,四川成都 611130
  • 折叠

摘要

为明确白及叶枯病病原菌种类,对白及叶枯病病原进行分离、纯化及rDNA-ITS序列分析鉴定其种属,并将纯化病原菌回接于白及植株,评价其致病性.结果表明,从白及患病样品上共分离6株真菌,其中有三株镰刀菌,分别为角化镰刀菌(Fusarium keratoplasticum)、尖孢镰刀菌(Fusarium oxysporum)、木贼镰刀菌(Fusarium equiseti);一株曲霉菌,为日本曲霉(Aspergillus japonicus);一株灰霉,为灰葡萄孢霉(Botrytis cinerea);一株毛霉,为易脆毛霉(Mucor fragilis).经致病性测定,发现上述6株真菌对白及均有致病性.

Abstract

In order to clarify the pathogenic bacteria species of Bletilla Striata leaf blight,the pathogen of B.Striata leaf blight was isolated,purified and identified by rDNA-ITS sequence analysis,the purified pathogen was grafted to B.Striata plants to evaluate its pathogenicity.The results showed that a total of 6 fungi strains were isolated from the diseased samples of B.striata,including three strains of Fusarium,namely:F.keratoplasticum,F.oxysporum and F.equiseti;a strain of Aspergillus japonicus;a strain of Botrytis cinerea;a strain of Mucor fragilis.According to the pathogenicity test,it was found that the above 6 strains of fungi were all pathogenic to B.Striata.

关键词

白及/叶枯病/病原鉴定/致病性

Key words

Bletilla Striata/leaf blight/pathogen identification/pathogenicity

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出版年

2024
四川林业科技
四川省林学会 四川省林业科学研究院

四川林业科技

影响因子:0.452
ISSN:1003-5508
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