四川林业科技2024,Vol.45Issue(4) :13-19.DOI:10.12172/202311260002

添加物对湖北花楸组培增殖苗生长和生根的影响分析

Effects of additives on the growth and rooting of tissue culture proliferating seedlings of Sorbus hupehensis

刘良梦 宋乾丽 黄振 刘欣 陈炙 李佳蔓 陈萍
四川林业科技2024,Vol.45Issue(4) :13-19.DOI:10.12172/202311260002

添加物对湖北花楸组培增殖苗生长和生根的影响分析

Effects of additives on the growth and rooting of tissue culture proliferating seedlings of Sorbus hupehensis

刘良梦 1宋乾丽 1黄振 1刘欣 1陈炙 1李佳蔓 1陈萍1
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作者信息

  • 1. 四川省林业科学研究院,森林和湿地生态恢复与保育四川省重点实验室,成都 610081
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摘要

以湖北花楸 5代以上的增殖芽苗为外植体,研究了提高不定芽诱导和生长的方法.结果表明,23种添加物中,琼脂糖和魔芋粉能显著提高芽苗生根率,其中添加魔芋粉的培养基生根效果最好.在含有 1 g·L-1魔芋粉的DKW+0.5 mg·L-1 NAA的生根培养基中,湖北花楸芽苗生根率由对照的 31.67%显著提升至 52.86%,当魔芋粉浓度增加至 2 g·L-1 时,生根率显著提升至 80.95%,并可诱导出大量不定根和侧根,平均每株芽苗有5.39条不定根,平均根长21 mm,6.19条侧根,平均侧根长6.7 mm.同时,芽苗茎段伸展,平均苗高可达4.4 cm.

Abstract

Using proliferating seedling of Sorbus hupehensis of more than 5 generations as explants,the method of improving the induction and growth of adventitious buds were studied.The results showed that among the 23 additives,agarose and konjac powder could significantly improve the rooting rate of sprouts,and the medium with added konjac powder had the best rooting effect.In the rooting medium of DKW+0.5 mg·L-1 NAA containing 1 g·L-1 konjac powder,the rooting rate of Sorbus hupehensis seedlings was significantly increased from 31.67%in the control to 52.86%.When the konjac powder concentration was increased to 2 g·L-1,the rooting rate increased significantly to 80.95%,and a large number of adventitious roots and lateral roots could be induced.Each sprout had an average of 5.39 adventitious roots,an average root length of 21 mm,and 6.19 lateral roots,with an average lateral root length of 6.7 mm.Meanwhile,the stem segments of the sprouts stretched,and the average seedling height could reach 4.3 cm.

关键词

湖北花楸/魔芋粉/增殖苗/不定根

Key words

Sorbus hupehensis/konjac flour/proliferating seedling/adventitious root

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基金项目

四川省林草科技创新团队项目(2023LCTD0208)

四川省基本科研业务费项目(2022JBKY12)

出版年

2024
四川林业科技
四川省林学会 四川省林业科学研究院

四川林业科技

影响因子:0.452
ISSN:1003-5508
参考文献量37
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