Influence of different light intensities on 1,8-cineole content in Camphora longepaniculata leaves
Camphora longepaniculata is a plant belonging to the Lauraceae family,and it is famous for its essential oil which is rich in 1,8-cineole.The 1,8-cineole has extensive economic value in the fields of food,chemical industry,and medical treatment.Plant growth process and secondary metabolism are regulated by changes in external environment,but there is no report on how light intensity affects the essential oil content and its composition changes,and how to regulate the expression of essential oil synthesis-related genes.In this experiment,C.longepaniculata leaves were used as materials to study the differences in the content of essential oil and 1,8-cineole in C.longepaniculata leaves under different light intensities,and to analyze the expression changes of eight key genes in the synthesis pathway of 1,8-cineole.The results showed that:(1)Different light intensities affected the leaf morphological development and endogenous hormone content;(2)There were significant differences in the content of essential oils and 1,8-cineole in leaves of C.longepaniculata under different lighting conditions.Light intensity was beneficial to the expression of key enzyme genes in the terpenoid synthesis pathway;(3)The expression of DXR,CMK,HGMR,GPPS,NPPS,CINS,and LIS were up-regulated in sun-side leaves of C.longepaniculata,while HDS were down-regulated.The purpose of this study was to deeply analyze the effect of light on the content of essential oil and 1,8-cineole in C.longepaniculata,and to provide a theoretical basis for further optimizing the cultivation conditions of C.longepaniculata,increasing the content of 1,8-cineole and regulating oil production by biotechnology.
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