Effect of ammonium nitrate substitutes on plant growth in MS medium
The safe and practical ammonium nitrate substitutes suitable for plant culture medium were screened to replace the original controlled drug ammonium nitrate(NH4NO3)as nitrogen source,which provided stable and easy-to-obtain medium component raw materials for plant tissue culture production.Nicotiana benthamiana and poplar(Populus×euramericana cv.''Nanlin95'')were used as experimental materials to study the effect of organic nitrogen sources and inorganic nitrogen sources[ammonium sulfate(NH4)2SO4 and diammonium hydrogen phosphate(NH4)2HPO4]substitutes on the formation of young roots,plant growth,biomass and chlorophyll content.In the study of ammonium nitrate substituion,there was no significant difference between tobacco growth on(NH4)2SO4 substitute medium and MS culture medium.However the growth of poplar on the appropriate concentration of(NH4)2SO4 medium was better than that on MS culture medium.In conclusion,(NH4)2SO4 can be used as a substitute for ammonium nitrate in MS medium.On the other hand,(NH4)2HPO4 could cause the medium to become turbid and was not suitable as a nitrogen source in MS culture medium.Additionally,plants grown on MS culture medium without added nitrogen source resulted in reduced chlorophyll accumulation,affecting plant growth.Results using tobacco and poplar as materials indicated that(NH4)2SO4 could be used as a substitute for NH4NO3 in MS culture medium for rapid production using plant tissue culture techniques.The result was also applicable to other plants using MS culture,and also had important reference value for all other culture media using NH4NO3 as nitrogen source.
万方数据
维普
