水酶法提取红花籽油工艺的研究
Study on Extraction Process of Safflower Oil by Aqueous Enzymatic
李倩 1赵丽 1马媛 1朱丹实 1王勃 1惠丽娟 1何余堂 1马涛 1刘贺1
作者信息
- 1. 渤海大学食品科学与工程学院,辽宁省食品安全重点实验室,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013
- 折叠
摘要
以红花籽为原料,在胰酶的最适pH条件下,采用中心组合设计对胰酶用量及料液比、酶解温度和时间进行优化,确定水酶法提取红花籽油最佳工艺为:酶添加量为305u/g,料液比1:6,提取温度45℃,提取时间为3h,红花籽油的最大提取率约为65.14％.气相色谱测定结果表明:水酶法得到的油脂由11种脂肪酸组成,其总含量高达90％以上,其中饱和脂肪酸以棕榈酸为主,不饱和脂肪酸以亚油酸和油酸为主,其中亚油酸含量最多,相对含量约为79％.
Abstract
Central Composite design was applied to optimize the aqueous enzymatic extraction parameters of safflower seed oil.The optimal hydrolysis condition were material liquid ratio of 1:6,trypsinase amount of 305u/g and temperature of 45℃,hydrolysis time of 3h as the discovery through response surface methodology.According to the optimal parameters,the validation extraction experiments have been carried out,and the maximal extraction rate of oil was 65.14％,the result was close to the predicted value.By using GC analysis,11 fatty acids were detected in the safflower oil,including saturated fatty acids (mainly palmitic acid) and unsaturated fatty acids (mainly oleic acid and linoleic acid).Among these fatty acids,the most abundant was linoleic acid with a relative content of 79％.
关键词
红花籽/水酶法/油得率/响应面分析/气相色谱Key words
safflower seed/aqueous enzymatic/oil yield/response surface methodology/GC引用本文复制引用
基金项目
国家自然科学基金面上项目(31471621)
国家自然科学基金青年基金(31201385)
辽宁省高等学校优秀人才支持计划(LR2014034)
锦州市科学技术计划项目(12A1B27)
出版年
2016
NETL
NSTL
维普
万方数据