Study on Subcritical Water Extraction of Polysaccharide from Ficus carica Linn
The purpose of this study was to establish the optimal conditions for extracting polysaccharide from Fi-cus carica Linn by subcritical water. Based on the results of a single-factors test such as extraction time, extrac-tion temperature, extraction pressure and liquid-solid ratio, the multinomial mathematical model of extracting polysaccharide by subcritical water was built with the method of response surface methodology(RSM), and then its extracting conditions under the subcritical water environment were optimized. The results indicated that the optimal conditions were found as follows: extraction time 17min, liquid-solid ratio 30mL/g, extraction temperature 150℃, and extraction pressure 1.5MPa, then verification test was carried out under the optimal extraction conditions, the extraction yield of polysaccharide reached up to 56.48%. There was no significant difference about the extraction yield of polysaccharide between the actual yield and predicted value, indicating the optimal conditions was reliable with the response surface analysis.
Ficus carica Linnsubcritical water extractionpolysaccharideresponse surface methodology