Selection and Optimization of Liquid Culture Medium for Industrialized Flammulina filiformis Strain
The aim was to study the different nutritional factors affecting liquid fermentation of Flammulina filiformis, optimize the yield of mycelium in liquid culture medium, and provide the basis for the development and utilization of F. filiformis in industrial production. Effects of 6 kinds of carbon source and nitrogen source in liquid culture medium were tested. Different carbon source, nitrogen source, inorganic salt and vitamin impact on the yield of mycelium were tested based on orthogonal design. The results showed that the effect of cornmeal, soluble starch and glucose was better than other tested carbon source, and the mycelium grew better in soybean power, bran and yeast powder. The main factors affecting the yield of mycelium were carbon source and nitrogen source. The liquid culture medium of F. filiformis was as follows: 3% soluble starch, 3% bran, 0.05% MgSO4 and 0.01% VB2.