Study on Immune Regulation Function of Zebrafish Treated with Pogostemon Perilla Tea
To study the regulatory effects of Pogostemon Perilla tea,a kind of anti-epidemic tea,on immune function of zebrafish with low immunity induced by vinorelbine tartrate.The zebrafish was intravenously injected with 0.2 mg/mL vinorelbine tartrate to induce hypoimmune model.According to random method,zebrafish were divided into normal control group,model control group,Pogostemon Perilla tea low-dose group(500 μg/mL),Pogostemon Perilla tea medium-dose group(1000 μg/mL),Pogostemon Perilla tea high-dose group(2000 μg/mL),30 tails per group.The effect of macrophage phagocytosis and T cell quantity in low immunity zebrafish was observed and analyzed by fluorescence microscopy.The experiment results showed that compared with model control group,the number of residual fluorescent microspheres in Pogostemon Perilla tea high-dose group(2 000 μg/mL)was significantly lower than that in model control group(P＜0.05).The fluorescence intensity of T cells in zebrafish in the low,medium,and high dose group of Pogostemon Perilla tea was significantly higher than that of model control group(P＜0.01).Pogostemon Perilla tea has the effect of promoting macrophage phagocytosis in zebrafish and improving T cell reduction.
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