Extraction Technology of Polysaccharide from Euryale ferox Salisb by Response Surface Methodology and Antioxidant Activity in Vitro
Single factor testing and response surface methodology were employed to optimize the extraction process of polysaccharide from Euryale ferox Salisb,and the in vitro antioxidant activities were further investigated.Taking the extraction rate of polysaccharide from Euryale ferox Salisb as the index,the effects of five single factors including extraction temperature,extraction time,solid-liquid ratio,pH and ethanol concentration on the extraction rate of Euryale ferox Salisb were investigated.Consequently,response surface method was performed to optimize these four main process parameters.The optimal extraction conditions of Euryale ferox Salisb polysaccharide were obtained as follows:the extraction temperature is 95℃,the extraction time 5.3 h,the solid-liquid ratio 1∶24,and the ethanol concentration 93%.The extraction rate of polysaccharide from Euryale ferox Salisb had the highest rate of 0.65%under the optimal extraction conditions.The results of in vitro antioxidant activity showed that in the range of 0.10～10.0 mg/mL,Euryale ferox Salisb polysaccharide had good antioxidant performance on the scavenging capacities of scavenging DPPH radical,superoxide anion free radical and hydroxyl radical with the results of 82.10%,58.91%and 71.07%respectively.Euryale ferox Salisb polysaccharide also had good total reducing power.The results of this study provided a theoretical basis for the creation of functional food and nutritional supplements based on Euryale ferox Salisb polysaccharide.
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