红曲霉菌孢子冻干保护剂的研究
Study on a Lyophilization Protective Agent for Spores of Monascus sp.
张蓓蓓 1冯霞 1朱英 1李峰 1张玲利 1陈麒名 1廖云生 1马卓元 1高礼红1
作者信息
- 1. 四川省食品发酵工业研究设计院有限公司,成都 611130;中国工业微生物菌种保藏管理中心西南菌种站,成都 611130;四川省微生物资源平台菌种保藏管理中心,成都 611130
- 折叠
摘要
以红曲霉菌为真空冷冻干燥菌种,通过单因素试验研究不同保护剂对红曲霉菌孢子存活率的影响,在此基础上,采用正交试验优化筛选出最优保护剂组合为蔗糖添加量14%、乳糖添加量3%、海藻糖添加量7%.验证试验得出,以该配方配制的保护剂冻干后红曲霉菌的存活率达到78.47%.
Abstract
Using Monascus sp.as a vacuum freeze-drying strain,the effect of different protective agents on the survival rate of Monascus spores was studied through a single factor experiment.Based on this,the optimal protective agent group was selected through orthogonal experiment optimization as sucrose addition 14%,lactose addition 3%,and trehalose addition 7%.The validation test showed that the survival rate of Monascus sp.after freeze-drying with the protective agent formulated with this formula reached 78.47%.
关键词
红曲霉菌/保护剂/存活率Key words
Monascus sp./protective agents/survival rate引用本文复制引用
基金项目
四川省重点研发计划(2021YFS0262)
微生物资源四川省科技资源共享服务平台项目(21PTKH0008)
出版年
2024