Preparation of Fluorescent Carbon Quantum Dot Molecularly Imprinted Polymers and Their Detection of Tetracyclines in Animal Foods
Tetracycline is a bacteriostatic broad-spectrum antibiotic,and its overuse in the farming industry can lead to animal residues,posing a potential hazard to consumer health.Therefore,the detection of tetracyclines in animal foods is crucial.In this experiment,water-soluble fluorescent Carbon Quantum Dots(CDs)were first prepared by microwave method.With CDs as the fluorescence source,tetracycline as template,3-aminopropyltriethoxysilane(APTES)as the functional monomer,and tetraethyl silicate(TEOS)as cross-linker,a novel,highly sensitive and selective Carbon Quantum Dots molecularly imprinted polymers(CDs@MIPs)were synthesized by sol-gel method for the detection of tetracycline(TC).The results of transmission electron microscopy and Fourier infrared spectroscopy confirmed the successful preparation of CDs@MIPs,which were synthesized with an imprinting factor(IF)of 2.28.Under the optimized experimental conditions,CDs@MIPs had a gradual decrease in fluorescence intensity in the range of 0.2～200 μmol/L,and showed a linear relationship in the concentration range of 0.2～1 μmol/L,with the lowest detection limit of 94 nmol/L.The selective experimental results showed that the prepared CDs@MIPs had a good selective recognition response to tetracycline.The recoveries of four animal foods(milk,egg,beef,chicken)were 96.00%～113.74%,96.94%～101.62%,102.29%～110.92%,102.58%～115.99%,and the RSD values were less than 4.16%,respectively.In summary,this method provides a new and effective method for the simple and rapid analysis of tetracycline in animal foods with complex matrices.
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