固相萃取-高效液相色谱法测定肉及肉制品中9种人工合成着色剂的研究
Determination of 9 Synthetic Colorants in Meat and Meat Products by Solid Phase Extraction-High Performance Liquid Chromatography
李丽鑫 1肖智超 2赵云龙 1王蕊 1马文思 1王文浩 3代佳和 2刘发山4
作者信息
- 1. 昆明市食品药品检验所,昆明 650000
- 2. 云南农业大学食品科学技术学院,昆明 650201
- 3. 红塔烟草(集团)有限责任公司,玉溪 653100
- 4. 弥渡县职业技术教育中心,大理 671000
- 折叠
摘要
建立同时测定肉及肉制品中9种水溶性人工合成着色剂含量的固相萃取-高效液相色谱法.方法采用氨水-乙腈-水溶液(1∶70∶29,v/v/v)提取,固相萃取小柱富集净化,多波长检测分析.肉及肉制品中9种水溶性人工合成着色剂在0.1～10 μg/mL浓度内线性关系良好,R2＞0.9999,检出限为0.02～0.20 mg/kg,回收率为75.0%～103.3%,相对标准偏差(RSD)为0.10%～1.73%.研究结果表明,本方法前处理操作简单,各种色素分离度和回收率高,检测结果准确,且实验重现性好.
Abstract
A solid phase extraction-high performance liquid chromatography method was established for the simultaneous determination of nine water-soluble synthetic colorants in meat and meat products.The method used extraction with ammonian-acetonitrile-aqueous solution(1∶70∶29,v/v/v),solid phase extraction column for enrichment and cleanup,and multi-wavelength detection to analyze.In meat and meat products,nine water-soluble synthetic colorants had good linear relationships at the concentrations of 0.1～10 μg/mL,the correlation coefficient R2＞0.9999,the limits of detection were 0.02～0.20 mg/kg,the recoveries were 75.0%～103.3%,and the relative standard deviations(RSD)were 0.10%～1.73%.The results showed that the method was simple in pre-treatment,high in the separation and recovery of various pigments,accurate in detection results,and good in experimental reproducibility.
关键词
固相萃取/高效液相色谱法/肉及肉制品/人工合成着色剂Key words
solid phase extraction/high performance liquid chromatography/meat and meat products/synthetic colorant引用本文复制引用
基金项目
2020年度云南省博士后科研基金资助项目()
出版年
2024
NETL
NSTL
万方数据