Optimization of Total Flavonoid Extraction from Helichrysum ledifolia and Assessment of Its Antioxidant Activity in Vitro
Helichrysum has significant antioxidant activity,and flavonoids are its main antioxidant components.In order to make full use of its resources,the optimal extraction process of total flavonoids was determined by response surface experiment in this study,and the antioxidant activity of Helichrysum was systematically evaluated.The results showed that the optimal extraction technology of total flavonoids was as follows:ethanol volume fraction 53％,extraction temperature 70℃,extraction time 1.0 h,solid-liquid ratio 1:50 g/mL.Under this process condition,the total flavonoid content was(338.43±0.71)mg/g.The results of antioxidant activity evaluation showed that total flavonoids had a significant scavenging effect on DPPH and ABTS+free radicals,and the concentration of total flavonoids had a significant positive correlation with its antioxidant capacity(P＜0.05),indicating a strong antioxidant capacity.This study not only provides reliable technological parameters for the efficient extraction of total flavonoids from Helichrysum,but also provides a reference for the comprehensive development and utilization of Helichrysum resources.
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