Study on Fermentation Technology and Extraction Conditions of Total Saponins from Trillium tschonoskii Maxim.by Aspergillus niger
Firstly,screen the optimal extraction method for total saponins from Trillium tschonoskii Maxim.Then by using UV and HPLC to compare and analyze the fermentation solution before and after fermentation,Box-Behnken response surface analysis was used to study the effects of Aspergillus niger inoculation amount,fermentation time,and fermentation temperature on the extraction rate of total saponins in Trillium tschonoskii Maxim.,which based on a single factor experiment.The results showed that:The best extraction method for total saponins in Trillium officinale is 75％ethanol reflux method,and the optimal fermentation conditions for total saponins of Trillium tschonoskii Maxim.were Aspergillus niger inoculant amount of 2.25％,fermentation time of 6 d and fermentation temperature of 27℃.Under these conditions,the extraction yield was 8.620％,which was basically consistent with the predicted results.
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