Study on Optimization of Activated Carbon Decolorization Process of L-valine Fermentation Broth
In this paper,the L-valine fermentation broth obtained by Escherichia coli fermentation was preliminarily filtered by membrane filtration,followed by decolorization using activated carbon,and the decolorization effect was evaluated by decolorization rate and L-valine retention rate.The decolorization effect of four different types of activated carbon on L-valine was investigated,and the experiment determined that the Yuanli HPC-07 activated carbon was selected as the decolorizing activated carbon,and the decolorization conditions were activated carbon dosage of 2％,decolorization temperature 65℃,fermentation broth pH 5.5,and decolorization time of 30 min.The color of the fermentation liquid was changed from brownish-yellow to a clarified colorless,and the retaining rate of L-valine was higher than 98.0％.The above research provides data reference for enterprises to produce high-quality L-valine.
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