Research on Control of L-arginine Fermentation Process
In order to improve the fermentation yield of L-arginine and realize the efficient industrial production of L-arginine,the fermentation process control of L-arginine-producing Escherichia coli was studied.The oxygen supply strategy is improved by adjusting the acceleration of glucose and ammonium sulfate flow.On the basis of continuous feeding,the process control method of dissolved oxygen feedback feeding and pH feedback regulation is established.Under this method,the level of acid production and the conversion rate of sugar and acid were significantly improved compared with continuous feeding,among which acid production was 91.2 g/L,a relative increase of 26.67％,and the conversion rate of sugar and acid was 46.65％,a relative increase of 23.97％.The average acid production was 90.4 g/L,and the average sugar and acid conversion rate was 47.02％after the validation of multiple batches of small-scale fermentation.The research provides an effective process control strategy for the production of L-arginine by Escherichia coli,and also provides a reference for the industrial production of L-arginine.
L-argininecontrol of fermentation processdissolved oxygen feedbackpH feedbackconversion rate
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