Study on Extraction Optimization and Stability of Betacyanins from Rice Leaves
In order to determine the quantitative analysis method of betacyanins as a reporter gene for detecting gene expression,this study investigated the extraction conditions and stability of betacyanins in rice leaves with the synthetic betacyanins synthesis gene using single-factor experiments and orthogonal experiments.The results indicated that the optimal extracting solvent for betacyanins in rice leaves is distilled water(pH 5.0),with the best extraction conditions being a material-liquid ratio of 1:30 g/mL,extraction temperature of 20℃,and extraction time of 30 minutes.After adding chloroform for secondary extraction,a clear maximum absorption peak appeared at 538 nm in rice leaves,which was consistent with the absorption peak of the standard beet red pigment.Stability tests showed that temperature,light exposure,and storage time all affect the stability of betacyanins.When stored at room temperature under natural light conditions for 1 day,the OD538 of betacyanins concentration decreased 37.3％.After 3 days of storage,the betacyanins concentration significantly decreased under all conditions,indicating that the betacyanins extracted under the conditions of this study should be stored at low temperature and protected from light,and the storage time should not be too long.
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