Safety Evaluation and Immunoregulatory Effect in Mice of YiYangJianJiu
To evaluate the safety of YiYangJianJiu and its effect on immune functions of mice.The safety of YiYangJianjiu was evaluated by acute toxicity test,30-day feeding toxicity test and genetic toxicity test.The immunity enhancement function of YiYangJianjiu was determined by measuring lymphocyte proliferative ability,DTH level,hemolytic plaques numbers,half hemolytic value of serum,monocyte-macrophage function and NK cell activity of mice.The results of acute toxicity test showed that the acute oral MTD of female and male mice of YiYangJianJiu was more than 22 400 mg/kg,which belonged to the actual non-toxic grade.The 30-day feeding toxicity test showed that the hematological and biochemical indexes of each dose group of YiYangJianJiu were within the normal range.No pathological changes related to the sample were found in the pathological histological examination of organs.The results of genotoxicity test showed that Ames test,sperm abnormality test and micronucleus test of bone marrow cells in mice were negative,and no teratogenic effect was observed.The results of functional studies showed that after oral administration of YiYangJianJiu at doses of 0.5,1.0 and 3.0 mL/(kg·bw·d)for 30 days,the results of lymphocyte proliferative ability,DTH level,hemolytic plaques numbers and half hemolytic value of serum of mice were negative,while the monocyte-macrophage and NK cell activities were positive.YiYangJianJiu is safe as a food supplement and can boost immunity of mice.
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