Analysis of Antibiotic Susceptibility and Resistance Genes in Multidrug-Resistant Commensal Bacteria Isolated from Mussels
This study employed multiple rounds of enrichment to screen for multidrug-resistant commensal bacteria in mussels.Antimicrobial resistance phenotypes were determined via the disk diffusion method,using second-generation sequencing technology for genome framework mapping sequencing and combining it with CARD database analysis of drug resistance genes.Growth kinetics curve was assessed by exposing the strains to various antimicrobial agents.The results showed that the isolated Y8 and Z2 symbiotic bacteria were highly multidrug-resistant,and they were resistant to 13 and 11 antimicrobial agents,respectively.These strains harbored resistance genes associated with β-lactams,and peptides,generally aligning with their resistance phenotypes.Furthermore,the growth kinetics of these strains displayed only limited susceptibility to the combined pressures of multiple drugs.In order to mitigate the further dissemination of resistance genes,it is necessary to enhance the management of antibiotic use in aquaculture.
万方数据
维普
