Study on the Optimization of Protease Production Process of Bacillus subtilis Engineering Bacteria WB800N/pHT43-npr Induced by Lactose
In order to explore a neutral protease fermentation process using low-cost,non-toxic lactose instead of IPTG as an inducer,this study used Bacillus subtilis engineering bacteria WB800N/pHT43-npr as the test strain,and compared the fermentation of lactose and IPTG inducers.On this basis,lactose was used as an inducer to optimize the fermentation effect of neutral protease produced by Bacillus subtilis engineering bacteria by response surface methodology.The results showed that the induction effect of adding 15.00 g/L lactose inducer was basically the same as that of adding 0.1%IPTG.The response surface methodology optimization of lactose induction method was carried out,obtaining the optimal fermentation conditions:culture temperature 35℃,fermentation time 47 h,initial pH 7.0,and adding inducers after 3 h fermentation.The final protease activity was 1079.31 U/mL,which was 14.22%higher than that induced by IPTG in the existing process.To a certain extent,it reduces the cost and removes the toxicity,which provides data support for the industrial application of neutral protease.
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