Optimization of L-Tyrosine Fermentation Process by Escherichia coli
L-tyrosine is an important essential amino acid,belonging to the aromatic amino acid,which is widely used in medicine,food and chemical industry.In this study,using the genetically engineered Escherichia coli TY03 strain as L-tyrosine producing bacteria.The effects of inoculation amount,fermentation temperature,dissolved oxygen,fermentation pH value and fermentation time on L-tyrosine production were studied by single factor and response surface experiments,and the suitable fermentation technology was determined.In the end,the pH was controlled at 6.8,dissolved oxygen was 31.2%,the fermentation temperature was 35.8℃,under such condition for 24 h,the L-tyrosine content reached 42.0 g/L.The study determined the appropriate fermentation process of strain TY03 producing L-tyrosine,and provided some theoretical basis for the production of L-tyrosine by microbial fermentation.
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