Optimization of Conditions for Whole Cell Transformation to Produce D-allulose
The production of D-allulose in a whole cell system has attracted attention due to its low cost and simple process.Studying the suitable conditions for the conversion of D-fructose into D-allulose in whole cells is of great significance in promoting the industrialization of D-allulose.This article explored the storage stability of whole cell enzymes as well as optimized and validated the conditions for the production of D-allulose from whole cells(including culture time,cell concentration,initial conversion pH,conversion temperature,and metal ions).The results showed that the whole cell enzyme had relatively strong storage stability,and the catalytic effect did not significantly decrease when placed at 40℃for 48h;At a concentration of 500g/L D-fructose substrate,the suitable conditions for whole cell transformation in production were cell concentration of 10 g/L,conversion temperature of 70℃,initial conversion pH equaling to natural pH,and conversion rate up to 31.55%,which was about 3%higher than before optimization.The conversion cycle was shortened to 4 h,and the production process was simplified,production efficiency was improved,and production costs were reduced.The research aims to provide an experimental basis for the industrial production of D-allulose and meet the needs of large-scale production.
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