Rapid Detection of Benzo[a]pyrene Residues in Drinking Water by Immunochromatography with Up-conversion Luminescence
A quantitative immunochromatographic test strip was established based on up-conversion luminescence materials for the rapid and quantitative detection of benzo[a]pyrene in drinking water,and the performance of the test strip was evaluated.Based on the competitive immunoassay model,and by optimizing the test strip elements,a benzo[a]pyrene test paper was developed.The performance of the strip was evaluated in terms of its standard curve,detection limit,quantitative limit,specificity,accuracy,etc.The results showed the regression equation R2 value of the standard curve of the developed test strip reached 0.983 5,the detection limit reached 1.007 ng/mL,the quantitative limit reached 1.938 ng/mL.The strip had no cross-reaction with structural analogs of benzo[a]pyrene,the recovery rate was 86.00%~108.47%.The results showed that the developed benzo[a]pyrene immunochromatographic test strip has the advantages of high sensitivity,good specificity,fast and simple detection,and can be applied to the detection of benzo[a]pyrene residues in drinking water.