Lactobacillus brevis HQ1-1 from Traditional Fermented Milk Source and Analysis of Its Genomic Characteristics and Glycometabolic Phenotype
In this paper,the basic characteristics of the genome and glycometabolic phenotype of Lactobacillus brevis HQ1-1 from traditional fermented milk were explored.The whole genome sequencing of Lactobacillus brevis HQ 1-1 was conducted by PacBio platform,and the functional classification and annotation of the genome were performed.The results showed that Lactobacillus brevis HQ1-1 carried a circular chromosome with a length of 2 376 708 bp and 7 plasmids DNA of different sizes,encoding 2 268 genes.There were 860 genes annotated by KEGG database involved in metabolic process,among them 142 genes were related to carbohydrate metabolism,accounting for 16.51％.Further,114 glycoside hydrolases genes were annotated using CAZy database,accounting for 45.24％of the total number of carbohydrate-related genes.According to OmniLog microarray phenotype chip analysis,Lactobacillus brevis HQ1-1 was found to have relatively higher efficiency of utilizing carbon sources like L-arabinose,D-xylose,D-ribose,L-lyxose.Therefore,the genome of Lactobacillus brevis HQ1-1 contains a large number of genes related to carbohydrate metabolism,possessing utilization capacity for various carbon source.The paper comprehensively elaborates the genome characteristics and metabolic phenotypes of Lactobacillus brevis HQ1-1,and provides novel lactic acid bacteria starter for the development of functional fermented food in the future.
traditional fermented milkLactobacillus brevis HQ1-1whole genome sequencingfunctional geneglycometabolic phenotype
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