传统发酵乳源短乳杆菌HQ1-1基因组特征与糖代谢表型分析
Lactobacillus brevis HQ1-1 from Traditional Fermented Milk Source and Analysis of Its Genomic Characteristics and Glycometabolic Phenotype
吴青海 1三月 2萨初拉 1郭煜 1王力伟 1王莉梅 1杨静 1其布日1
作者信息
- 1. 内蒙古自治区农牧业科学院生物技术研究所,呼和浩特 010031
- 2. 内蒙古商贸职业学院餐饮食品系,呼和浩特 010070
- 折叠
摘要
该文探究传统发酵乳源短乳杆菌(Lactobacillus brevis)HQ1-1的基因组基本特征和糖代谢表型.经PacBio平台对短乳杆菌HQ1-1进行全基因组测序,并对基因组进行功能分类和注释.结果显示,短乳杆菌HQ1-1包含1条长度为2376 708 bp的环状染色体和7个大小不同的质粒DNA,编码2268个基因.通过KEGG数据库注释到参与代谢过程的基因有860个,其中,碳水化合物代谢相关基因的数量为142个,占比为16.51％.进一步用CAZy数据库注释到了糖苷水解酶类基因114个,占碳水化合物相关基因总数的45.24％.通过OmniLog微阵列表型芯片分析发现,短乳杆菌HQ1-1对L-阿拉伯糖、D-木糖、D-核糖、L-来苏糖等碳源的利用效率相对更高.因此,发酵乳源短乳杆菌HQ1-1基因组中包含碳水化合物代谢相关基因的数量较多,能够利用多种碳源.该文全面阐述了短乳杆菌HQ1-1基因组特征和代谢表型,为今后开发功能性发酵食品提供新型乳酸菌发酵剂.
Abstract
In this paper,the basic characteristics of the genome and glycometabolic phenotype of Lactobacillus brevis HQ1-1 from traditional fermented milk were explored.The whole genome sequencing of Lactobacillus brevis HQ 1-1 was conducted by PacBio platform,and the functional classification and annotation of the genome were performed.The results showed that Lactobacillus brevis HQ1-1 carried a circular chromosome with a length of 2 376 708 bp and 7 plasmids DNA of different sizes,encoding 2 268 genes.There were 860 genes annotated by KEGG database involved in metabolic process,among them 142 genes were related to carbohydrate metabolism,accounting for 16.51％.Further,114 glycoside hydrolases genes were annotated using CAZy database,accounting for 45.24％of the total number of carbohydrate-related genes.According to OmniLog microarray phenotype chip analysis,Lactobacillus brevis HQ1-1 was found to have relatively higher efficiency of utilizing carbon sources like L-arabinose,D-xylose,D-ribose,L-lyxose.Therefore,the genome of Lactobacillus brevis HQ1-1 contains a large number of genes related to carbohydrate metabolism,possessing utilization capacity for various carbon source.The paper comprehensively elaborates the genome characteristics and metabolic phenotypes of Lactobacillus brevis HQ1-1,and provides novel lactic acid bacteria starter for the development of functional fermented food in the future.
关键词
传统发酵乳/短乳杆菌HQ1-1/全基因组测序/功能基因/糖代谢表型Key words
traditional fermented milk/Lactobacillus brevis HQ1-1/whole genome sequencing/functional gene/glycometabolic phenotype引用本文复制引用
出版年
2024
NETL
NSTL
万方数据