The quality of fresh Ruditapes philippinarum is susceptible to deterioration,which is more likely to occur under the relatively rudimentary conditions of intra-city e-commerce delivery.The focus of this study is the effect of temperature and delivery time on the quality of fresh Ruditapes philippinarum during simulated intra-city e-commerce delivery process,and the quality indicators,such as survival rate,total bacterial colony count,water retention capacity,TVB-N content,TBA value,color difference,texture characteristics,moisture distribution,and MRI characteristics,etc,were examined at three delivery temperatures(10 ℃,25 ℃,and 35 ℃).The results showed that the increased of delivery temperature and time significantly reduced the survival rate,and browned of clam flesh,while increased the total bacterial count,TVB-N content,TBA value,cooking loss rate,and juice loss rate.Meanwhile,the proportion of immobile water decreased while the proportion of free water increased,and the red-yellow area and cavities on the MRI pseudo-color image were expanded,resulting in deteriorated texture.It indicated that delivery condition at 10 ℃ and 2 h helps to maintain the quality of fresh Ruditapes philippinarum.This study can provide a reference for the optimization of intra-city delivery conditions for fresh Ruditapes philippinaru.