Research on the Process Optimization of Lyophilized of Lacticaseibacillus paracasei HCS17-040 and Its Lipolysis Efficacy
This research optimized the lyophilized process of Lacticaseibacillus paracasei HCS17-040 and investigated its lipolysis efficacy.The production process of lyophilized Lacticaseibacillus paracasei HCS 17-040 powder was optimized by single factor test and orthogonal test.At the same time,using zebrafish experiment,the change of fluorescence intensity of yolk sac fat of zebrafish was analyzed after taking lyophilized powder,and its lipolysis efficacy was explored.The results showed that after optimization,the optimal process was as follows:the proportion of bacteria and protective agent was 1∶3.0g/g,the pre-freezing time was 2.0 h,and the pre-freezing temperature was-25 ℃.Under these conditions,the viable bacteria number after freeze-drying was 5.4×1011 CFU/g.When the concentration of Lacticaseibacillus paracasei HCS 17-040 was 1.25 mg/mL,the fluorescence intensity of yolk sac fat of zebrafish was the lowest,which reached a significantly low level compared with blank control(P＜0.05).It showed that Lacticaseibacillus paracasei HCS 17-040 effectively breaks down fat and can provide raw materials for the development of other weight loss products.
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