Selective Breeding and Transformation Determination of Trehalose-producing Strain
In this paper,the strains with trehalose production potential were selected from the nature,and a certain amount of strains were first obtained by the primary screening medium with high screening pressure,and then the transformation effect of the trehalose production of the target strain was determined by high-performance liquid chromatography(HPLC).A high-quality strain HS-202006211109-117 which can produce trehalose was screened from the hot spring sample HS-202006211109 by the established screening method,and the amount of trehalose produced by it was 3.73 mg/mL at 45 ℃ for 6h,which was better than that report in the literature as 0.56 g/L by 3 h transformation.After Gram stained,biochemical identification and fluorescence quantitative PCR,the strains were identified as Bacillus thermotolerans.The strains had excellent genetic stability and environmental tolerance.This paper selectively bred high-yield strains that can transform maltose to produce trehalose,provided technical support for the autonomy of key technology for trehalose production.
NETL
NSTL
万方数据
