微波辅助水解/酶比色法快速测定特殊医学用途配方食品中胆碱
Rapid Determination of Choline in Food for Special Medical Purpose by Enzyme Colorimetry Using Microwave-assisted Hydrolysis
崔梦琪 1罗璇 1娄湘琴 2梅英杰 1牟丹 1安迈瑞 1李政 1张贞理1
作者信息
- 1. 淄博市检验检测计量研究总院,淄博 255000
- 2. 淄博市淄川区检验检测中心,淄博 255000
- 折叠
摘要
该文利用微波辅助水解技术提取特殊医学用途配方食品中胆碱,并采用酶比色法进行测定.胆碱受到胆碱氧化酶的作用释放过氧化氢(H2O2),在过氧化物酶的作用下,与苯酚和4-氨基安替比林反应生成有色物质,利用分光光度法进行外标法定量.对标准曲线、水解试剂、微波辅助加热条件进行了优化,并将确定的最佳方法在不同基质的样品中进行了实验验证.选择1.0mol/L盐酸(HCl)溶液在100 ℃下微波辅助水解样品5 min,测定结果和加标回收率与水浴加热水解的国家标准方法相当,微波辅助水解前处理方法简单、快速,准确度高,适用于批量测定特殊医学用途配方食品中胆碱.
Abstract
This paper extracted choline from food for special medical purpose using microwave-assisted hydrolysis,and detected the choline content by enzyme colorimetry.Choline was catalyzed by choline oxidase with subsequent discharge of hydrogen peroxide(H2O2).In the presence of peroxidase,hydrogen peroxide reacted with phenol and 4-aminoantipyrine and colored substances were formed.The external standard method was used for quantitative analysis of choline by spectrophotometry.Standard curve,the hydrolysis-solvent and microwave-assisted hydrolysis heating condition were optimized.The determined optimum method was experimentally verified in the samples of different matrices.Selecting samples with 1.0 mol/L HCl solution for microwave-assisted hydrolysis at 100℃ for 5 min,the analysis results and spiked recovery rates agreed quite well with those obtained by the national standard method for water bath heating hydrolysis.The pretreatment method of microwave-assisted hydrolysis is simple,rapid and highly accurate,and it is applicable for batch detection of choline in food for special medical purpose.
关键词
微波辅助水解/酶比色法/胆碱/特殊医学用途配方食品Key words
microwave-assisted hydrolysis/enzyme colorimetry/choline/food for special medical purpose引用本文复制引用
出版年
2024
NETL
NSTL
万方数据