摘要
为了探究提取长茎葡萄蕨藻多糖的最优工艺及抗氧化活性,对目前已有的多糖提取方法进行筛选,并采用单因素实验和响应面实验的方法,对料液比、提取温度、提取时间、木瓜蛋白酶添加量和提取次数这 5 个因素进行优化.结果显示,添加木瓜蛋白酶提取长茎葡萄蕨藻多糖的方法最高效便捷,且当料液比 1∶40,提取温度 50℃,提取时间 3h,提取次数 2 次,以及木瓜蛋白酶添加量为 2.0%时,长茎葡萄蕨藻多糖提取率相对较高,可达到 41.24%±0.09%.进一步的实验结果显示,长茎葡萄蕨藻多糖对DPPH和ABTS自由基均具有良好的清除活性,其IC50 值分别为 2.32 和 0.67 mg/mL.研究表明,使用优化后的木瓜蛋白酶酶解法能有效提高长茎葡萄蕨藻多糖的提取率,且长茎葡萄蕨藻多糖具有良好的抗氧化活性.本研究可为长茎葡萄蕨藻多糖的开发利用提供理论基础和参考依据.
Abstract
Caulerpa lentillifera is a kind of perennial large edible seaweed,which is also known as"sea grape"because of its upright stem globule glittering and translucent,similar in appearance to grapes.C.lentillifera is also one of the most suitable seaweeds for human consumption.It is rich in nutrients and contains a variety of amino acids,vitamins,polysaccharides and fatty acids needed by the human body.Among them,polysaccharide is the most important active component in C.lentillifera.According to research,the crude polysaccharide content of C.lentillifera accounts for 34.00%-64.97%of its dry weight,which is higher than that of most edible algae,such as Scagassum,Hizikia fusifarme and Undaria pinnatifida.At present,the main extraction methods for polysacchar-ides of C.lentillifera are water extraction,alkali extraction and ultrasonic-assisted extraction,and the extraction rate is 3.22%-11.30%,but there are still a large amount of polysaccharides that remains un-extracted.In order to develop a mild,efficient and low-cost extraction process of polysaccharides from C.lentillifera,and to explore the antioxidant activity of the polysaccharide,we screened five existing extraction methods of polysaccharides from C.lentillifera.Through single factor experiment and response surface experiment,the ratio of material to solution,extraction temperature,extraction time,amount of papain and extraction times were optimized,and the optimum process parameters were obtained.Polysaccharide concentration was determined by anthrone sulfate method in the experiment.In addition,we investigated the scavenging activity of the polysaccharide on DPPH and ABTS free radicals.The results showed that adding papain is the most efficient method to extract polysaccharides from C.len-tillifera.The order of the factors affecting the extraction rate of polysaccharide from C.lentillifera is as follows:ratio of material to solution>extraction temperature>extraction time,and the ratio of material to solution showed an extremely significant influence on the extraction rate of the polysaccharide(P<0.001).The best extraction para-meters after optimization were as follows:ratio of material to solution is 1∶45,extraction temperature 60℃,extraction time 3 h,extraction count 2 times,the addition amount of papain 2.0%.Under this condition,the extrac-tion rate of polysaccharides can reach 41.24%±0.09%.In addition,polysaccharides from C.lentillifera had good scavenging activities on DPPH and ABTS free radicals,and their IC50 values were 2.32 and 0.67 mg/mL,respect-ively.This study shows that the optimized papain enzymatic hydrolysis can improve the extraction rate of polysac-charides from C.lentillifera effectively,among which the ratio of material solution is the most important factor affecting this method.Besids,the polysaccharides of C.lentilliferahave good antioxidant activity.
基金项目
广西壮族自治区科技计划(桂科AB18221113)
国家虾产业技术体系建设专项(GK AB18221113)
National Shrimp Industry Technology System Construction Project(CARS-48)
国家科技期刊平台
NSTL
维普
万方数据