Mesenteric fat weight(MFW)is a representative index used to reflect body lipid level in Ctenopharyn-godon idella.However,the traditional measurements by artificial scrape and weighing are time-consuming,labor intensive and having high operating errors,especially when many samples are to be handled.To avoid such limita-tions,this study developed a rapid and sensitive method for the quantitation of mesenteric fat utilizing the lyso-chrome dye oil red O.The initial operation procedures included:fish anesthetization,sample collection of visceral mass,PIT-tag implantation,sample fixation,sample dehydration,oil red O staining.After the above steps,mesen-teric fat could be specifically stained by oil red O dye,and all the samples were stained uniformly.After sample extraction and absorbance measurement of extraction liquid,the weight of oil red O extracted from the stained sample could be accurately obtained according to the standard curve of oil red O(y=0.0276x+0.0403,R2=0.9997).The oil red O weights were used as MFWs in this study,and had good consistency with the fat weights quantified by the conventional scraping method(r = 0.80).Furthermore,data analysis showed that the coefficient of variation for MFW(24.49%)was greater than that for body weight(8.93%)in the cultured population of C.idella(n = 200),indicating a significant potential for its genetic improvement.Correlation and cluster analysis showed that MFW was positively correlated with all the observed traits,of which visceral weight(VW)had the highest correlation coefficient of 0.60(P＜0.01),and was clustered into one group.The results were as expected that both MFW and VW belonged to viscera-related indexes.Multiple linear regression analysis showed that morphological indexes could explain only a small amount of variation for MFW(R2 = 0.20),indicating that the regression prediction was unsatisfactory,and direct measurement remained an effective approach.This study provided an accurate quantitat-ive method for genetic improvement of body lipid traits in C.idella.
关键词
草鱼/肠系膜脂肪组织/油红O/沉积量/定量分析
Key words
Ctenopharyngodon idella/mesenteric adipose tissue/oil red O/deposition amount/quantitative ana-lysis
维普
万方数据