Algae-lysing activity of Vibrio mediterranei 117-T6
Many algal pathogens have a broad spectrum of algae-lysing activity.In order to verify whether Vibrio mediterranei 117-T6(Vm 117-T6)has broad-spectrum algicide properties,this paper observed its algal lysis on Heterosigma akashiwo,Pleurochrysis carterae,Dirateria inornata and Prorocentrum donghaiense,and the char-acteristics of the algae-lysing substances were analyzed.In this study,Vm 117-T6,a pathogenic bacterium for the yellow spot disease of Pyropia,showed a broad-spectrum algae-lysing activity.It exhibited strong algae-lysing activity to H.akashiwo,P.carterae,D.inornata and P.donghaiense,which significantly reduces the chlorophyll content of these microalgaes.Vm 117-T6 can also reduce the motility of H.akashiwo within 40 min,inhibit photo-synthesis,cause algal lysis and produce white flocculent precipitates after infection over the 120 min.Vm 117-T6 can degrade carrageenan,but cannot degrade pectin,cellulose and chitin.The live bacterium,its intracellular and extracellular extracts showed strong microalgae-lysing activity.The extracellular algae-lysing compounds were easily soluble in water,insoluble in petroleum ether and ethyl acetate,which indicated strong polarity.It was res-istant to high temperature,and was not easy adsorbed by activated carbon.Ethanol treatment reduced the lysing-algae activity.There were a large amount of ABC transporter,outer membrane protein,flagellin and a small amount of toxin in the key differential proteins under conventional and susceptible conditions.The results indicate that Vm 117-T6 does not dissolve algae through cell wall degradation,and the virulence effectors of Vm 117-T6 contain endotoxins,and the proteins related to the transport,secretion and cell adhesion may play an important role in the virulence of Vm 117-T6.This paper provides evidence for the analysis of the algae-lysing mechanism of this strain,and also lays a foundation for the prevention and control of this strain.
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