Allograft inflammatory factor-1 stimulates leukocyte viability and inflammatory mediator release from Ctenopharyngodon idella
To clarify the role of Ctenopharyngodon idella allograft inflammatory factor-1(AIF-1)in host immune response,AIF-1 expression profile in peripheral blood leukocytes was investigated with lipopolysaccharide expos-ure using western blot.Then,the effect of AIF-1 on cell viability was assayed with a CCK-8 kit,cell apoptosis was detected by flow cytometer,ATP production,mitochondrial membrane potential,reactive oxygen species and nitric oxide were determined with the corresponding kit.Finally,the levels of inflammatory cytokine tumor nec-rosis factor-alpha(TNF-α),interleukin 1β(IL1β),interleukin 6(IL6)were measured using ELISA kits.The results shown that lipopolysaccharide stimulated AIF-1 secretion from leukocytes.Furthermore,C.idella AIF-1 induced leukocyte proliferation,and increased mitochondrial membrane potential,subsequently promoted ATP production,prevented leukocyte apoptosis.In addition,it provoked inflammatory mediator reactive oxygen species,nitric oxide,inflammatory cytokine TNF-α,IL1β,IL6 released from leukocytes.These results indicated AIF-1 was a potential stimulator for leukocyte viability and associated with C.idellaimmune response.
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