首页|脑室注射葡萄糖对翘嘴鳜摄食和糖代谢的影响

脑室注射葡萄糖对翘嘴鳜摄食和糖代谢的影响

扫码查看
为探究鳜下丘脑葡萄糖感知系统的存在和对摄食的影响,实验以翘嘴鳜为研究对象,对鳜分别进行脑室注射生理盐水(对照组)、2-DG(葡萄糖代谢拮抗剂,100 mg/kg)和葡萄糖(10 mg/kg),探究注射后 3、6 和 12h不同时间点鳜摄食及糖代谢的响应机制.结果显示,脑室注射葡萄糖显著抑制鳜的摄食,而不同时间点鳜血浆葡萄糖含量无显著差异.脑室注射葡萄糖显著诱导下丘脑葡萄糖激酶基因gk(glucokinase,6 h),表明鳜下丘脑葡萄糖感知体系的存在.且脑室注射葡萄糖显著促进可卡因和苯丙胺调节转录肽基因 cart(cocaine and amphetamine regulated transcript,3 h)的表达量,这可能与鳜mTOR(mammalian target of rapamycin)在下丘脑(12 h)和肝脏(6 h)中的基因表达水平的显著上调有关.另外,脑室注射葡萄糖显著诱导鳜肝脏gk(6 h)和pk(pyruvate kinase,3 h)的表达,促进糖酵解,表明脑室通过感知的葡萄糖水平促进分解代谢为机体供能.综上,本研究首次通过脑室注射葡萄糖探究鳜下丘脑葡萄糖感知系统的存在,同时发现鳜下丘脑对葡萄糖脑室注射存在响应.且脑室注射葡萄糖可能通过对gk的调控影响AMPK/mTOR通路,调控机体食欲相关基因表达进而抑制摄食.研究结果为翘嘴鳜对饲料碳水化合物的高效利用和摄食调控研究提供理论依据.
Effects of intracerebroventricular injection of glucose on feeding and glucose metabolism in mandarin fish(Siniperca chuatsi)
Diets containing high levels of carbohydrate could inhibit the feeding in many fish species,but the rel-evant mechanisms behind this regulation are not clear.The mandarin fish(Siniperca chuatsi),a typical carnivor-ous fish,exhibits an apparent anorexia due to its extensive intolerance to dietary carbohydrates,which limits its value as a cultured species.To investigate the existence of a glucose sensing system in the hypothalamus of S.chuatsi and its effect on feed intake,intracerebroventricular(ICV)injections of saline(control),2-DG(glucose metabolism antagonist,100 mg/kg)and glucose(10 mg/kg)were performed,exploring the impacts on feeding and glucose metabolism at 3,6 and 12 h.The results showed that ICV of glucose significantly inhibited the feed intake of mandarin fish,while the plasma glucose levels remained constant at different time points.The expression of glucokinase(gk)were significantly induced at 6 h after ICV administration of glucose,indicating the presence of a glucose-sensing system in the hypothalamus of mandarin fish.ICV administration of glucose significantly pro-moted the mRNA levels of the cocaine and amphetamine-regulated transcriptional peptide(cart)at 3 h,which may be related to the significant upregulation of mTOR in the hypothalamus(12 h)and liver(6 h)of mandarin fish.In addition,ICV administration of glucose significantly induced the expression of gk(6 h)and pk(3 h)in the liver of mandarin fish to promote glycolysis,suggesting the promotion of catabolism for energy supply through per-ceived glucose levels in the hypothalamus.In conclusion,this study demonstrates for the first time that gk in the hypothalamus can response to the glucose level,suggesting the potential existence of glucose sensing system in the hypothalamus of mandarin fish,and that ICV administration of glucose regulates the AMPK/mTOR pathway through gk-induced glucose sensing,which in turn regulates the expression of appetite-related genes to inhibit feed intake.The present study would provide a theoretical basis for research on the carbohydrate utilization and feeding regulation in mandarin fish.

Siniperca chuatsiglucose-sensing systemintracerebroventricular injectionfeeding regulationgluc-ose metabolism

曾妍芝、李红燕、王广军、谢骏、田晶晶、龚望宝、夏耘、张凯、李志斐、郁二蒙

展开 >

上海海洋大学,水产科学国家级实验教学示范中心,上海 201306

中国水产科学研究院珠江水产研究所,农业农村部亚热带热带水产资源利用与养殖重点实验室,广东广州 510380

中国水产科学研究院海南创新研究院,海南三亚 572000

翘嘴鳜 葡萄糖感知 脑室注射 摄食调控 糖代谢

广东省基础与应用基础研究基金中国水产科学研究院基本科研业务费专项国家现代农业产业技术体系建设专项

2023A15150100082022SJ-XK1CARS-45-21

2024

水产学报
中国水产学会

水产学报

CSTPCD北大核心
影响因子:1.148
ISSN:1000-0615
年,卷(期):2024.48(4)
  • 49