温度升高和游离余氯对泥蚶的氧化胁迫效应

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中文摘要：为了研究核电站温排水污染对水生动物长期胁迫的影响，实验通过室内模拟核电站温排水的游离余氯残留和温升效应，研究了不同温度和不同游离余氯浓度耦合下对泥蚶的氧化胁迫效应。设置水温为19和29 ℃，游离余氯浓度分别为0。33和6。66 mg/L，进行为期20d的胁迫实验。结果显示:①单一温升条件下，温度升高可以显著影响泥蚶ROS水平。温度越高，ROS水平越高。②0。33 mg/L浓度组泥蚶ROS水平和DNA损伤在第20天时显著升高，19和29 ℃下分别为对照组的2。2、2。9倍和1。8、2。2倍;细胞活性、SOD活性、ATP酶活性均下降;6。66 mg/L浓度组ROS水平在第20天时显著上升，19和29 ℃ 下分别为对照组的6。2、3。6倍，DNA损伤增加，细胞活性、血细胞吞噬率和ATP酶活性下降，SOD酶活性在第20天、29 ℃时降至最小值(36。4±8。4)U/(mg·prot)。③双因素方差分析结果显示，水温和游离余氯对泥蚶ROS水平、DNA损伤、血细胞吞噬活性、ATP酶活性具有显著交互作用。研究表明，游离余氯能够显著增加泥蚶ROS水平和DNA损伤，温度升高和游离余氯复合暴露对泥蚶造成更大的胁迫压力，影响了其机体内氧化响应相关生理过程，余氯排放海域夏季相比冬季会对泥蚶产生更大的氧化胁迫。本研究可为温度升高和游离余氯复合污染中的海洋生物氧化胁迫毒性研究提供参考，并且为生态风险评估提供科学依据。

外文标题：Oxidative stress effect of temperature rise and free residual chlorine on Tegillarca granosa

外文摘要：To investigate the effects of warm waste water from nuclear power plants on the long-term stress of aquatic animals,the influence of free residual chlorine on the oxidative pressure of Tegillarca granosa under dif-ferent temperature conditions were studied through laboratory simulation.Water temperatures were set as 19 and 29 ℃,and two different gradients of free residual chlorine concentration were set as 0.33 mg/L and 6.66 mg/L.The results showed that:① water temperature can significantly affect the ROS level.The higher the temperature,the higher the ROS level.② the ROS level and DNA damage of T.granosa in 0.33 mg/L group were increased significantly on day 20,which were 2.2,2.9 times and 1.8,2.2 times higher than those in the control group at 19 and 29 ℃,respectively;the cell activity,ATPase activity and SOD enzyme activity decreased significantly;the ROS level in the 6.66 mg/L group increased significantly on day 20,which was 6.2 and 3.6 times higher than that in the control group at 19 ℃ and 29 ℃,respectively;the DNA damage increased;the cell activity,hemocyte pha-gocytosis rate and ATPase activity decreased;SOD enzyme activity decreased to the minimum value of(36.4±8.4)U/(mg prot)at 29 ℃ on day 20.③ two-factor analysis of variance showed that water temperature and free resid-ual chlorine had significant interaction on the parameters of ROS level,DNA damage,hemocyte phagocytosis rate and ATPase activity.The results indicate that free residual chlorine can significantly increase the ROS level and the DNA damage of T.granosa.The combined exposure of water temperature and residual chlorine,which caused greater stress pressure on T.granosa,affected the physiological processes related to oxidative stress.In the resid-ual chlorine discharge area,greater oxidative stress would be faced by T.granosa in summer than in winter.This study can give a reference for studying oxidative stress toxicity of marine organisms exposed to warm water com-bined with residual chlorine,and provide scientific basis for ecological risk assessment.

外文关键词：

Tegillarca granosawarm drainage pollution of nuclear power planttemperature risefree residual chlorineoxidative responseROSDNA damage

作者：

陈琳、滕爽爽、胡高宇、肖国强、黄晓林

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作者单位：

浙江省海洋水产养殖研究所,浙江省近岸水域生物资源开发与保护重点实验室,温州市海洋生物遗传育种重点实验室,浙江温州 325005

浙江海洋大学,国家海洋设施养殖工程技术研究中心,浙江舟山 316022

关键词：

泥蚶核电站温排水温度升高游离余氯氧化响应活性氧 DNA损伤

基金：

浙江省重点研发计划现代农业产业技术体系建设专项台州市重大农业科技"揭榜挂帅"研发攻关计划

项目编号：

2019C02045CARS-49NYJBGS202203

出版年：

2024

DOI：

10.11964/jfc.20211213256

水产学报

中国水产学会

水产学报

CSTPCD北大核心

影响因子：1.148

ISSN：1000-0615

年,卷(期)：2024.48(6)

参考文献量38